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Immortalization of paired human brown and white adipose progenitor cells

• Paired human A41hBAT-SVF and A41hWAT-SVF cells are available at ATCC
  ATCC® CRL-3385™
  ATCC® CRL-3386™
• References:
  • Xue R, LynesMD, Dreyfuss JM, Shamsi F, Schulz TJ, ZhangH, Huang TL, Townsend KL, Li Y, Takahashi H, Weiner LS, White AP, Lynes MS, Rubin LL, Goodyear LJ, Cypess AM, Tseng YH. Clonal analyses and gene profiling identify genetic biomarkers of human brown and white preadipocyte thermogenic potential. Nature Medicine 2015; 21(7):760-768. PMCID: PMC4496292. PDF
  • Kriszt R, Arai S, Itoh H, Lee MH, Goralczyk AG, Ang XM, Cypess AM, White AP, Shamsi F, Xue R, Lee JY, Lee SC, Hou Y, Kitaguchi T, Sdhaharan T, Ishiwata S, Lane EB, Chang YT, Tseng YH^#, Suzuki M^#, Raghunath M^#. Optical visualisation of thermogenesis in stimulated single-cell brown adipocytes. Sci Rep 2017; 7(1): 1383. ^#Corresponding author. PMCID: PMC5431191. PDF
• Addgene, Tseng Lab Plasmids – https://www.addgene.org/Yu-Hua_Tseng/

Protocols:

• Protocol for differentiation of mouse brown preadipocytes (2009)
• Protocols for generation of immortalized human brown and white preadipocyte cell lines. Shamsi F, Tseng YH. Methods MolBiol2017;1566:77-85. PMCID: PMC5870121. PDF
• Isolation of Stromal Vascular Fraction (SVF) from mouse brown adipose tissue (BAT) for single cell RNA-seq
• Protocol for Analyzing Mitochondrial Function in Brown Adipocytes with a Bioenergetic Analyzer. Darcy J, Wang CH, Tseng YH. Methods Mol Biol. 2020; 2138:289-296. PDF
• Measurement of heat production (thermogenesis) in cells using ERthermAC dye_microplate method. Link
• Signaling Lipidomic Analysis of Thermogenic Adipocytes. Kodani SD, Bussberg V, Narain NR, Kiebish MA, Tseng YH. Methods Mol Biol. 2022;2448:251-271. doi: 10.1007/978-1-0716-2087-8_16. PMID: 35167102. PDF

Datasets:

• microRNA expression in C3H10T1/2 cells treated with BMP7 vs vehicle (Accession: GSE71157)
• Human brown and white preadipocyes clonal dynamics predict themorgenic potential and function(Accession: GSE68544)
• Human and mouse lipidomics, ST001215, ST001207, ST001213, ST001214 (under the Project ID: PR000811). (Accession: ST001206)
• Single-cell RNA-seq of brown adipose tissue from mice housed at thermoneutrality, room temperature, and cold conditions. (Accession: GSE160585)

Online lipidomic viewer:

• Human plasma lipidomics with 1 hour of cold challenge
• Mouse brown fat lipidomics with 7 days of cold exposure
• Mouse white fat lipidomics with 7 days of cold exposure
• Mouse brown and white fat lipidomics from Ames dwarf and control mice

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