Publications

Selected Publications

2023

Tsuji T, Zhang Y, Tseng YH. Generation of Brown Fat-Specific Knockout Mice Using a Combined Cre-LoxP, CRISPR-Cas9, and Adeno-Associated Virus Single-Guide RNA System. J Vis Exp. 2023 Mar 24;(193). doi: 10.3791/65083. PMID: 37036212.

2022

Kempf E, Landgraf K, Stein R, Hanschkow M, Hilbert A, Abou Jamra R, Boczki P, Herberth G, Kühnapfel A, Tseng YH, Stäubert C, Schöneberg T, Kühnen P, Rayner NW, Zeggini E, Kiess W, Blüher M, Körner A. Aberrant expression of agouti signaling protein (ASIP) as a cause of monogenic severe childhood obesity. Nat Metab. 2022 Dec 19. doi: 10.1038/s42255-022-00703-9. Epub ahead of print. PMID: 36536132. PDF

Tseng YH. Adipose tissue in communication: within and without. Nat Rev Endocrinol. 2022 Dec 16:1–2. doi: 10.1038/s41574-022-00789-x. Epub ahead of print. PMID: 36526875; PMCID: PMC9756712. PDF

Rahbani JF, Scholtes C, Lagarde DM, Hussain MF, Roesler A, Dykstra CB, Bunk J, Samborska B, O’Brien SL, Tripp E, Pacis A, Angueira AR, Johansen OS, Cinkornpumin J, Hossain I, Lynes MD, Zhang Y, White AP, Pastor WA, Chondronikola M, Sidossis L, Klein S, Kralli A, Cypess AM, Pedersen SB, Jessen N, Tseng YH, Gerhart-Hines Z, Seale P, Calebiro D, Giguère V, Kazak L. ADRA1A-Gαq signalling potentiates adipocyte thermogenesis through CKB and TNAP. Nat Metab. 2022 Nov 7. doi: 10.1038/s42255-022-00667-w. Epub ahead of print. PMID: 36344764. PDF

Xiao H, Bozi LHM, Sun Y, Riley CL, Philip VM, Chen M, Li J, Zhang T, Mills EL, Emont MP, Sun W, Reddy A, Garrity R, Long J, Becher T, Vitas LP, Laznik-Bogoslavski D, Ordonez M, Liu X, Chen X, Wang Y, Liu W, Tran N, Liu Y, Zhang Y, Cypess AM, White AP, He Y, Deng R, Schöder H, Paulo JA, Jedrychowski MP, Banks AS, Tseng YH, Cohen P, Tsai LT, Rosen ED, Klein S, Chondronikola M, McAllister FE, Van Bruggen N, Huttlin EL, Spiegelman BM, Churchill GA, Gygi SP, Chouchani ET. Architecture of the outbred brown fat proteome defines regulators of metabolic physiology. Cell. 2022 Nov 2:S0092-8674(22)01318-6. doi: 10.1016/j.cell.2022.10.003. Epub ahead of print. PMID: 36334589. PDF

Sakaguchi M, Okagawa S, Okubo Y, Otsuka Y, Fukuda K, Igata M, Kondo T, Sato Y, Yoshizawa T, Fukuda T, Yamagata K, Cai W, Tseng YH, Sakaguchi N, Kahn CR, Araki E. Phosphatase protector alpha4 (α4) is involved in adipocyte maintenance and mitochondrial homeostasis through regulation of insulin signaling. Nat Commun. 2022 Oct 14;13(1):6092. doi: 10.1038/s41467-022-33842-4. PMID: 36241662; PMCID: PMC9568526. PDF

Park K, Li Q, Lynes MD, Yokomizo H, Maddaloni E, Shinjo T, St-Louis R, Li Q, Katagiri S, Fu J, Clermont A, Park H, Wu IH, Yu MG, Shah H, Tseng YH, King GL. Endothelial Cells Induced Progenitors Into Brown Fat to Reduce Atherosclerosis. Circ Res. 2022 Jul 8;131(2):168-183. doi: 10.1161/CIRCRESAHA.121.319582. Epub 2022 Jun 1. PMID: 35642564; PMCID: PMC9308716. PDF

Sugimoto S, Mena HA, Sansbury BE, Kobayashi S, Tsuji T, Wang CH, Yin X, Huang TL, Kusuyama J, Kodani SD, Darcy J, Profeta G, Pereira N, Tanzi RE, Zhang C, Serwold T, Kokkotou E, Goodyear LJ, Cypess AM, Leiria LO, Spite M, Tseng YH. Brown adipose tissue-derived MaR2 contributes to cold-induced resolution of inflammation. Nat Metab. 2022 Jun;4(6):775-790. doi: 10.1038/s42255-022-00590-0. Epub 2022 Jun 27. PMID: 35760872. PDF

Townsend KL, Pritchard E, Coburn JM, Kwon YM, Blaszkiewicz M, Lynes MD, Kaplan DL, Tseng YH. Silk Hydrogel-Mediated Delivery of Bone Morphogenetic Protein 7 Directly to Subcutaneous White Adipose Tissue Increases Browning and Energy Expenditure. Front Bioeng Biotechnol. 2022 May 12;10:884601. doi: 10.3389/fbioe.2022.884601. PMID: 35646839; PMCID: PMC9135469. PDF

Tsuji T, Bussberg V, MacDonald AM, Narain NR, Kiebish MA, Tseng YH. Transplantation of Brown Adipose Tissue with the Ability of Converting Omega-6 to Omega-3 Polyunsaturated Fatty Acids Counteracts High-Fat-Induced Metabolic Abnormalities in Mice. International Journal of Molecular Sciences. 2022; 23(10):5321. https://doi.org/10.3390/ijms23105321. PDF

Rosina M, Ceci V, Turchi R, Chuan L, Borcherding N, Sciarretta F, Sánchez-Díaz M, Tortolici F, Karlinsey K, Chiurchiù V, Fuoco C, Giwa R, Field RL, Audano M, Arena S, Palma A, Riccio F, Shamsi F, Renzone G, Verri M, Crescenzi A, Rizza S, Faienza F, Filomeni G, Kooijman S, Rufini S, de Vries AAF, Scaloni A, Mitro N, Tseng YH, Hidalgo A, Zhou B, Brestoff JR, Aquilano K, Lettieri-Barbato D. Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue. Cell Metab. 2022 Mar 15:S1550-4131(22)00088-2. doi: 10.1016/j.cmet.2022.02.016. Epub ahead of print. PMID: 35305295. PDF

Kodani SD, Bussberg V, Narain NR, Kiebish MA, Tseng YH. Signaling Lipidomic Analysis of Thermogenic Adipocytes. Methods Mol Biol. 2022;2448:251-271. doi: 10.1007/978-1-0716-2087-8_16. PMID: 35167102. PDF

Lynes MD, Carlone DL, Townsend KL, Breault DT, Tseng YH. Telomerase reverse transcriptase expression marks a population of rare adipose tissue stem cells. Stem Cells 2022. DOI: https://doi.org/10.1093/stmcls/sxab005. PDF

Gupta A, Shamsi F, Altemose N, Dorlhiac GF, Cypess AM, White AP, Yosef N, Patti ME, Tseng YH, Streets A. Characterization of transcript enrichment and detection bias in single-nucleus RNA-seq for mapping of distinct human adipocyte lineages. Genome Res. 2022 Jan 18. doi: 10.1101/gr.275509.121. Epub ahead of print. PMID: 35042723. PDF

2021

Shamsi F, Wang CH, Tseng YH. The evolving view of thermogenic adipocytes – ontogeny, niche and function. Nat Rev Endocrinol. 2021 Oct 8. doi: 10.1038/s41574-021-00562-6. Epub 2021. PMID: 34625737. PDF

Cero C, Lea HJ, Zhu KY, Shamsi F, Tseng YH, Cypess AM. β3-Adrenergic receptors regulate human brown/beige adipocyte lipolysis and thermogenesis. JCI Insight 2021 Jun 8;6(11):139160. doi: 10.1172/jci.insight.139160. PMID: 34100382. PDF

Lundh M, Altıntaş A, Tozzi M, Fabre O, Ma T, Shamsi F, Gerhart-Hines Z, Barrès R, Tseng YH, Emanuelli B. Cold-induction of afadin in brown fat supports its thermogenic capacity. Sci Rep. 2021 May 7;11(1):9794. doi: 10.1038/s41598-021-89207-2. PMID: 33963248. PMCID: PMC8105362. PDF

Kodani S, Tseng YH. Narrating the story ARC of adipose tissue aging. Developmental Cell 2021;56(10):1359-1360. DOI: 0.1016/j.devcel.2021.04.020. PMID: 34004148. PDF

Löffler MC, Betz MJ, Blondin DP, Augustin R, Sharma AK, Tseng YH, Scheele C, Zimdahl H, Mark M, Hennige AM, Wolfrum C, Langhans W, Hamilton BS, Neubauer H. Challenges in tackling energy expenditure as obesity therapy – from preclinical models to clinical application. Mol Metab 2021. DOI:10.1016/j.molmet.2021.101237. PDF

Shamsi F, Piper M, Ho L-L, Huang TL, Gupta A, Streets A, Lynes MD, Tseng YH. Vascular smooth muscle-derived TRPV1-positive progenitors are a source of cold-induced thermogenic adipocytes. Nat Metab 2021 Apr 12. DOI: 10.1038/s42255-021-00373-z. Epub ahead of print. PMID: 33846638. PDF

Angueira AR, Sakers AP, Holman CD, Cheng L, Arbocco MN, Shamsi F, Lynes MD, Shrestha R, Okada C, Batmanov K, Susztak K, Tseng YH, Liaw L, Seale P. Defining the lineage of thermogenic perivascular adipose tissue. Nat Metab 2021 Apr 12. DOI: 10.1038/s42255-021-00380-0. Epub ahead of print. PMID: 33846639. PDF

Schlein C, Fischer AW, Sass F, Worthmann A, Tödter K, Jaeckstein MY, Behrens J, Lynes MD, Kiebish MA, Narain NR, Bussberg V, Darkwah A, Jespersen NZ, Nielsen S, Scheele C, Schweizer M, Braren I, Bartelt A, Tseng YH, Heeren J, Scheja L. Endogenous Fatty Acid Synthesis Drives Brown Adipose Tissue Involution. Cell Rep. 2021 Jan 12;34(2):108624. PMID: 33440156. PDF

Farnaz S, Tseng YH, CR Kahn. Adipocyte Microenvironment: Everybody in the Neighborhood Talks about the Temperature. Cell Metab 2021;33(1):1-6. DOI: 10.1016/j.cmet.2020.12.012. PDF

2020

Wolfs D, Lynes MD, Tseng YH, Pierce S, Bussberg V, Darkwah A, Tolstikov V, Narain NR, Rudolph MC, Kiebish MA, Demerath EW, Fields DA, Isganaitis E. Brown fat-activating lipokine 12,13-diHOME in human milk is associated with infant adiposity. J Clin Endocrinol Metab. 2020 Nov 2:dgaa799. DOI: 10.1210/clinem/dgaa799. Online ahead of print. PMID: 33135728. PDF

Blaszkiewicz, M, Wood E, Koizar S, Willows J, Anderson R, Tseng YH, Godwin J, Townsend K. The involvement of neuroimmune cells in adipose innervation. Mol Med. 2020. PDF

Leiria LO, Tseng YH. Lipidomics of brown and white adipose tissue: Implications for energy metabolism. Biochim Biophys Acta Mol Cell Biol Lipids. 2020 Oct;1865(10):158788. doi: 10.1016/j.bbalip.2020.158788. Epub 2020 Aug 4. PMID: 32763428; PMCID: PMC7484152. PDF

Wang, CH, Lundh M, Fu A, Kriszt R, Huang TL, Lynes MD, Leiria LO, Shamsi F, Darcy J, Greenwood BP, Narain NR, Tolstikov V, Smith KL, Emanuelli B, Chang YT, Hagen S, Danial NN, Kiebish MA, Tseng YH. CRISPR-engineered human brown-like adipocytes prevent diet-induced obesity and ameliorate metabolic syndrome in mice. Sci Transl Med 2020;12(558):1-14. DOI: 10.1126/scitranslmed.aaz8664. PDF

Darcy, J, Tseng YH. The link between stress and IL-6 is heating up. Cell Metab 2020; 32:152-153. DOI:10.1016/j.cmet.2020.07.011. PDF

Knights A, Wu J, Tseng YH. The heating microenvironment: intercellular crosstalk within thermogenic adipose tissue. Diabetes 2020; 69(8):1599-1604. PMID: 32690661. PDF

Lynes MD, Tseng YH. Commentary on: “The Presence of Active Brown Adipose Tissue Determines Cold-Induced Energy Expenditure and Oxylipin Profiles in Humans.” J Clin Endocrinol Metabo 2020; 105(8):dgaa339. PMCID: PMC7304926. PDF

Darcy J, Fang Y, McFadden S, Leiria LO, Dreyfuss JM, Bussburg V, Tolstikov V, Greenwood B, Narain NR, Kiebish MA, Bartke A, Tseng YH. Integrated metabolomics reveals altered lipid metabolism in adipose tissue in a model of extreme longevity. GeroScience 2020. DOI: 10.1007/s11357-020-00221-0. PDF

Pirouz M, Wang CH, Liu Q, Ebrahimi A, Shamsi F, Tseng YH, and Gregory R. The Perlman syndrome DIS3L2 exoribonuclease safeguards endoplasmic reticulum-targeted mRNA translation and calcium ion homeostasis. Nat Commun 2020;11(1):2619. PMCID: PMC7250864. PDF

Ramirez AK, Dankel SN, Rastegarpanah B, Cai W, Xue R, Crovella M, Tseng, YH, Kahn CR, Kasif S. Single-cell transcriptional networks in differentiating preadipocytes suggest drivers associated with tissue heterogeneity. Nat Commun. 2020;11(1):2117. PMCID: PMC7192917. PDF

De Jesus DF, Orime K, Kaminska D, Kimura T, Basile G, Wang CH, Haertle L, Riemens R, Brown N, Hu J, Männistö V, Silva A, Dirice E, Tseng YH, Haaf T, Pihlajamäki J, Kulkarni RN. Parental metabolic syndrome epigenetically reprograms offspring hepatic lipid metabolism in mice. J Clin Invest. 2020;130(5):2391–2407. PMCID: PMC7190992. PDF

Shwartz Y, Gonzalez-Celeiro M, Chen CL, Pasolli HA, Sheu SH, Fan S, Shamsi F, Assaad S, Lin E, Zhang B, Tsai PC, He M, Tseng YH, Lin SJ, and Hsu YC. Cell types promoting goosebumps form a niche to regulate hair follicle stem cells. Cell 2020. DOI: 10.1016/j.cell.2020.06.03. PMCID: PMC7540726. PDF

Darcy J, Wang CH, Tseng YH. Analyzing Mitochondrial Function in Brown Adipocytes with a Bioenergetic Analyzer. Methods Mol Biol. 2020; 2138:289-296. PDF

Sato M, Tsuji T, Yang K, Ren X, Dreyfuss JM, Huang TL, Wang CH, Shamsi F, Leiria LO, Lynes MD, Yau KW, Tseng YH. Cell-autonomous light sensitivity via Opsin3 regulates fuel utilization in brown adipocytes. PLOS Biology, 2020. PMCID: PMC7034924. PDF

Shamsi F, Xue R, Huang TL, Lundh M, Liu Y, Leiria LO, Lynes MD, Kempf E, Wang CH, Sugimoto S, Nigro P, Landgraf K, Schulz T, Li Y, Emanuelli B, Kothakota S, Williams LT, Jessen N, Pedersen SB, Böttcher Y, Blüher B, Körner A, Goodyear LJ, Mohammadi M, Kahn CR, Tseng YH. FGF6 and FGF9 regulate UCP1 expression independent of brown adipogenesis. Nat Comm 2020 11(1): 1421. PMCID: PMC7078224. PDF   

2019

Lynes MD, Kodani SD, Tseng YH. Lipokines and Thermogenesis. Endocrine Society, 2019. DOI: 10.1210/en.2019-00337.  PDF

Darcy J, Tseng YH. ComBATing aging-does increased brown adipose tissue activity confer longevity? Geroscience, 2019. PMCID: PMC6702504. PDF

Lundh M, Petersen PSS, Isidor MS, Kazoka-Sørensen DNM, Plucinska K, Shamsi F, Ørskov C, Tozzi M, Brown EL, Andersen E, Ma T, Müller U, Barres R, Kristiansen VG, Gerhart-Hines Z, Tseng YH, Emanuelli B. Afadin is a scaffold protein repressing insulin action via HDAC6 in adipose tissue. EMBO Reports 2019. PMCID: PMC6680131. PDF

Leiria LO, Wang CH, Lynes MD, Yang K, Shamsi F, Sato M, Sugimoto S, Chen EY, Bussberg V, Narain NR, Sansbury BE, Darcy J, Huang TL, Kodani SD, Sakaguchi M, Rocha AL, Schulz TJ, Bartelt A, Hotamisligil GS, Hirshman MF, Leyen KV, Goodyear LJ, Bluher M, Cypess AM, Kiebish MA, Spite M, Tseng YH. 12-lipoxygenase regulates cold adaptation and glucose metabolism by producing the omega-3 lipid 12-HEPE from brown fat. Cell Metabolism; 2019; S1550-4131(19)30374-2. PMID: 31353262. PDF

Takahashi H, Alves CRR, Stanford KI, Middelbeek RJW, Nigro P , Ryan RE, Xue R, Sakaguchi M, Lynes MD, So K, Mul JD, Lee MY , Balan E, Pan H, Dreyfuss JM, Hirshman MF, Azhar M, Hannukainen JC, Nuutila P, Kalliokoski KK, Nielsen S, Pedersen BK, Kahn CR, Tseng YH, Goodyear LJ. TGF-β2 is an exercise-induced adipokine that regulates glucose and fatty acid metabolism. Nat Metab 2019; 1:291-303. PDF

2018

Gupta MK, De Jesus DF, Kahraman S, Valdez IA, Shamsi F , Yi L, Swensen AC, Tseng YH, Qian WJ, Kulkarni RN. Insulin receptor-mediated signaling regulates pluripotency markers and lineage differentiation. Mol Metab 2018. PDF

Arai S, Kriszt R, Harada K, Looi LS, Matsuda S, Wongso D, Suo S, Ishiura S, Tseng YH, Raghunath M, Ito T, Tsuboi T, Kitaguchi T. RGB-color intensiometric indicators visualize spatiotemporal dynamics of ATP in single cells. AngewChemInt Ed Engl 2018; 57(34):10873-10878. PDF

Lynes, M. D., Shamsi, F., Sustarsic, E. G., Leiria, L. O., Wang, C. H., Su, S. C., Huang, T. L., Gao, F., Narain, N. R., Chen, E. Y., Cypess, A. M., Schulz, T. J., Gerhart-Hines, Z., Kiebish, M. A., Tseng, Y. H. (2018). Cold-Activated Lipid Dynamics in Adipose Tissue Highlights a Role for Cardiolipin in Thermogenic Metabolism. Cell Reports, 24(3), 781-790. PMCID: PMC6117118 PDF

Lynes M, Tseng YH . Deciphering adipose tissue heterogeneity. Ann. N.Y. Acad. Sci 2018 PMCID: PMC5788721. PDF 

Sustarsic EG, Ma T, Lynes MD, Larsen M, Karavaeva L, Havelund JF, Nielsen CH, Jedrychowski MP, Moreno-Torres M, Lundh M, Plucinska K, Jespersen NZ, Grevengoed TJ, Kramar B, Peics J, Hansen JB, Shamsi F, Forss I, Neess D, Keipert S, Wang J, Stohlmann K, Brandslund I, Christensen C, Jørgensen ME, Linneberg A, Pedersen O, Kiebish MA, Qvortrup K, Han X, Pedersen BK, Jastroch M, Mandrup S, Kjær A, Gygi SP, Hansen T, Gillum MP, Grarup N, Emanuelli B, Nielsen S, Scheele C, Tseng YH, Færgeman NJ, Gerhart-Hines Z. Cardiolipin Synthesis in brown and beige fat mitochondria is essential for systemic energy homeostasis. Cell Metab2018;28(1):159-174.e11. PMCID: PMC6038052. PDF

Stanford K, Lynes M, Takahashi H, Baer LA, Arts PJ, May FJ, Lehnig AC, Middelbeek RJW, Richard JJ, So K, Chen EY, Gao F, Narain NR, Distefano G, Shettigar VK, Hirshman MF, Ziolo MT, Kiebish MA, Tseng YH, Coen PM, Goodyear LJ. 12,13-diHOME: An exercise-induced lipokine that increases skeletal muscle fatty acid uptake. Cell Metab 2018; 1;27(5):1111-1120.e3. PMCID: PMC5935136. PDF

Bartelt A, Widenmaier SB, Schlein C, Johann K, Goncalves RLS, Eguchi K, Fischer AW, Parlakgul G, Snyder NA, Nguyen TB, Bruns OT, Franke D, Bawendi MG, Lynes MD, Leiria LO, Tseng YH, Inouye KE, Arruda AP, Hotamisligil GS. Brown adipose tissue thermogenic adaptation requires Nrf1-mediated proteasomal activity. Nat Med 2018; 24(3):292-303. PMCID: PMC5839993. PDF

Gao F, McDaniel J, Chen EY, Rockwell HE, Nguyen C, Lynes MD, Tseng YH, Sarangarajan R, Narain NR, Kiebish MA. Adapted MS/MSALL Shotgun Lipidomics Approach for Analysis of Cardiolipin Molecular Species. Lipids. 2018;53(1):133-142. PMID: 29488636. PDF

2017

Ramirez AK, Lynes MD, Shamsi F, Xue R, Tseng YH, Kahn CR, Kasif S, Dreyfuss JM. Integrating extracellular flux measurements and genome-scale modeling reveals novel differences between brown and white adipocytes. Cell Reports 2017;21(11):3040-3048. PDF

Townsend KL, Madden C, Blaszkiewicz M, McDougall L, Tupone D, Lynes MD, Mishina Y, Yu P, Morrison S, Tseng YH. Re-establishment of energy balance in a mouse model with POMC neuron deletion of BMPR1A. Endocrinology 2017;158(12):4233-4245. PMCID: PMC5711385. PDF

Shamsi F, Zhang H, and Tseng YH. MicroRNA regulation of brown adipogenesis and thermogenic energy expenditure. Frontiers in Endocrinology. 2017; 8(205): 1-7. PMCID: PMC5572399. PDF 

Shamsi F, Tseng YH.  Protocols for generation of immortalized human brown and white preadipocyte cell lines. Methods Mol Biol 2017;1566:77-85. PMCID: PMC5870121. PDF 

Hiraike Y, Waki H, Yu J, Nakamura M, Miyake K, Nagano G, Nakaki R, Suzuki K, Kobayashi H, Yamamoto S, Sun W, Aoyama T, Hirota Y, Ohno H, Oki K, Yoneda M, White AP, Tseng YH, Cypess AM, Larsen TJ, Jespersen NJ, Scheele C, Tsutsumi S, Aburatani H, Yamauchi T, and Kadowaki T. NFIA co-localizes with PPARγ and transcriptionally controls the brown fat gene program. Nat Cell Bio 2017;19(9):1081-1092. PMCID: PMC5885759. PDF

Hou Y, Kitaguchi T, Kriszt R, Tseng YH, Raghunath. Ca2+-associated triphasic pH changes in mitochondria during brown adipocyte activation. Mol Metab 2017; 6(8): 797-808. PMCID: PMC5518710. PDF 

Mo Q, Salley J, Roshan T, Baer LA, May FJ, Jaehnig EJ, Lehnig AC, Guo X, Tong Q, Nuotio-Antar AM, Shamsi F, Tseng YH, Stanford KI, Chen MH. Identification and characterization of a supraclavicular brown adipose tissue in mice. JCI Insight 2017;2(11): 93166 . PMCID: PMC5453704. PDF

Lynes M, Leiria L, Lundh M, Bartelt A, Shamsi F, Huang TL, Takahashi H, Hirshman MF, Schlein C, Lee A, Baer LA, May FJ, Gao F, Narain NR, Chen EY, Kiebish MA, Cypess AM, Blüher M, Goodyear LJ, Hotamisligil GS, Stanford KI, Tseng YH. The cold-induced lipokine 12,13-diHOME promotes fatty acid transport into brown adipose tissue.  Nat Med 2017; 23(5): 631-637. PMCID: PMC6484428. PDF  

Kriszt R, Arai S, Itoh H, Lee MH, Goralczyk AG, Ang XM, Cypess AM, White AP, Shamsi F, Xue R, Lee JY, Lee SC, Hou Y, Kitaguchi T, Sdhaharan T, Ishiwata S, Lane EB, Chang YT, Tseng YH # , Suzuki M # , Raghunath M # . Optical visualisation of thermogenesis in stimulated single-cell brown adipocytes. Sci Rep 2017; 7(1): 1383. ^#Corresponding author. PMCID: PMC5431191. PDF

2016

Gao F, McDaniel J, Chen EY, Rockwell H, Lynes MD, Tseng YH, Sarangarajan R, Narain NR and Kiebish MA. Monoacylglycerol analysis using MS/MSALL quadruple time of flight mass spectrometry. Metabolites 2016, 6, 25. PMCID: PMC5041124. PDF

Zhu Y, Gao Y, Tao C, Shao M, Huang W, Yao T, Johnson JA, Liu T, Cypess AM, Gupta O, Holland W, Gupta RK, Spray DC, Tanowitz HB, Cao L, Lynes MD, Tseng YH, Elmquist JK, Williams KW, Lin HV, Scherer PE. Connexin-43 mediates white adipose tissue beiging by facilitating the propagation of sympathetic neuronal signals. Cell Metab 2016; 24: 420–433. PMCID: PMC5024720. PDF

Schulz TJ, Graja A, Huang TL, Xue R, An D, Poehle-Kronawitter S, Lynes MD, Tolkachov A, O’Sullivan LE, Hirshman MF, Schupp M, Goodyear LJ, Mishina Y., Tseng YH. Loss of BMP receptor type 1A in adipose tissue prevents age-related impairment of glucose homeostasis and insulin sensitivity. Diabetologia 2016; 59(8):1769-77. PMCID: PMC4930470. PDF 

Jeong HW, Choi RH, McClellan JL, Piroli GG, Frizzell N, Tseng YH, Goodyear LJ, Koh HJ. Tribbles 3 inhibits brown adipocyte differentiation and function by suppressing insulin signaling. Biochem Biophys Res Commun 2016; 2016; 470(4):783-91 PMCID: PMC4768059. PDF

2015

Unsar AM, Mooney B, Hempel N, Corr D, Tseng YH, Xie Y. 3D brown adipogenesis to create “Brown-Fat-in-Microstrands”. Biomaterials 2015; 75:123-134. PMCID: PMC4644497. PDF

Zhang X, Tian Y, Zhang H, Kavishwar A, Lynes MD, Brownell AL, Sun H, Tseng YH, Moore A, Ran C. Curcumin analogues as selective fluorescence imaging probes for brown adipose tissue and monitoring browning. Sci Rep. 2015; DOI: 10.1038/srep13116. PMC4534785. PDF

Zhang H, Guan M, Townsend KL, Huang TL, An D, Yan X, Xue R, Schulz TJ, Winnay J, Mori M, Hirshman MF, Kristiansen K, Tsang JS, White AP, Cypess AM, Goodyear LJ, Tseng YH. MicroRNA-455 regulates brown adipogenesis via a novel HIF1an-AMPK-PGC1α signaling network. EMBO Reports 2015; 16(10):1378-93. PMCID: PMC4766451. PDF

Xue R, Lynes MD, Dreyfuss JM, Shamsi F, Schulz TJ, ZhangH, Huang TL, Townsend KL, Li Y, Takahashi H, Weiner LS, White AP, Lynes MS, Rubin LL, Goodyear LJ, Cypess AM, Tseng YH. Clonal analyses and gene profiling identify genetic biomarkers of the thermogenic potential of human brown and white preadipocytes. Nat Med 2015; 21(7):760-768. PMCID: PMC4496292. PDF

Xue R, Lynes MD, Dreyfuss JM, Shamsi F, Schulz TJ, Zhang H, Huang TL, Townsend KL, Li Y, Takahashi H, Weiner LS, White AP, Lynes MS, Rubin LL, Goodyear LJ, Cypess AM Tseng YH. Functional and Clonal Analyses of Human Brown and White Fat Progenitors Identify Markers that Predict Thermogenic Capacity of Mature Adipocytes. Nat Med 2015; 21(7):760-768. PMCID: PMC4496292 PDF

Shinoda K, Luijten IH, Hasegawa Y, Hong H, Sonne SB, Kim M, Xue R, Chondronikola M, Cypess AM, Tseng YH, Nedergaard J, Sidossis LS, Kajimura S. Genetic and functional characterization of clonally derived adult human brown adipocytes. Nat Med. 2015; 21(4):389-94. PMCID:PMC4427356   PDF   

Lynes MD, Schulz TJ, Pan AJ, Tseng YH. Disruption of insulin signaling in Myf5-expressing progenitors leads to marked paucity of brown fat, but normal muscle development. Endocrinology 2015; epub ahead of print.  PMCID: PMC4398768. PDF

Stanford KI, Middelbeek RJ, Townsend KL, Lee MY, Takahashi H, So K, Hitchcox KM, Markan KR, Hellbach K, Hirshman MF, Tseng YH, Goodyear LJ. A Novel Role for Subcutaneous Adipose Tissue in Exercise-Induced Improvements in Glucose Homeostasis. Diabetes 2015; epub ahead of print. PMCID: PMC4439563 PDF

2014

Lau AM, Tseng YH, Schulz TJ. Adipogenic fate commitment of muscle-derived progenitor cells: isolation, culture, and differentiation. Methods in Molecular Biology 2014; 1213:229-43. PMID: 25173387 PDF

Lynes MD, Tseng YH. Unwiring the transcriptional heat circuit. Proceedings of the National Academy of Sciences 2014; 111(40):14318-9. PMCID: PMC4209983 PDF

Huang P, Schulz TJ, Beauvais A, Tseng YH, Gussoni E. Intramuscular adipogenesis is inhibited by myo-endothelial progenitors with functioning Bmpr1a signaling. Nat Comm 2014; 5;5:4063. PMCID: PMC4084855  PDF

Kong X, Banks A, Liu T, Kazak L, Rao RR, Cohen P, Wang X, Yu S, Lo JC, Tseng YH, Cypess AM, Xue R, Kleiner S, Kang S, Spiegelman BM, Rosen ED. IRF4 is a key thermogenic transcriptional partner of PGC-1α. Cell 2014; 158(1):69-83. PMCID: PMC4116691  PDF  

Ussar S, Dankel S, Boucher J, Lee K, Haering MF, Kleinridders A, Xue R, Macotela Y, Cypess AM, Tseng YH, Mellgren G, Kahn CR. ASC-1, PAT2, and P2RX5 are cell surface markers for white, beige, and brown adipocytes. Sci Transl Med 2014; 6, 247ra103. PMCID: PMC4356008   PDF  

Castiglioni A, Hettmer S, Lynes MD, Rao TN, Tchessalova D, Sinha I, Lee BT, Tseng YH, Wagers AJ. Isolation of progenitors that exhibit myogenic/osteogenic bipotency in vitro by fluorescence-activated cell sorting from human fetal muscle. Stem Cell Reports 2014; 2(1)92-106. PMCID: PMC3966115.  PDF  

Lee JH, Bhang DH, Beede A, Huang TL, Stripp BR, Bloch KD, Wagers AJ, Tseng YH, Ryeom S, Kim CF. Lung stem cell differentiation in mice directed by endothelial cells via a BMP4-NFATc1-thrombospondin-1 axis. Cell 2014; 156(3):440-55. PMCID: PMC3951122  PDF  

Mwangi SM, Nezami BG, Obukwelu B, Anitha M, Marri S, Fu P, Epperson MF, Le NA, Shanmugam M, Sitaraman SV, Tseng YH, Anania FA, Srinivasan S. Glial cell line-derived neurotrophic factor protects against high fat diet-induced obesity. Am J Physiol Gastrointest Liver Physiol 2014; 306(6):G515-25. PMCID: PMC3949027  PDF  

Townsend KT, Tseng YH. Brown fat fuel utilization and thermogenesis. Trends Endocrinol Metab 2014; 25(4):168-177. PMCID: PMC3972344 (** This article was selected as the 10 best article published in TEM in 2014)  PDF  

2013

Cypess AM, Doyle AN, Sass CA, Huang TL, Mowschenson PM, Rosen HN, Tseng YH, Palmer EL III, Kolodny GM. Quantification of human and rodent brown adipose tissue function using 99mTc-methoxyisobutylisonitrile SPECT/CT and 18F-FDG PET/CT. J Nuc Med 2013; 19(5):635-9. PMCID: PMC4012367  PDF 

Schulz TJ, Tseng YH. Systemic control of brown fat thermogenesis: integration of peripheral and central signals. Ann. N.Y. Acad. Sci 2013; 1302(1):35-41. PMCID: PMC3805713  PDF  

Townsend KL, An D, Lynes MD, Huang TL, Zhang, H, Goodyear LJ, Tseng YH. Increased mitochondrial activity in BMP7-treated brown adipocytes, due to increased CPT1- and CD36-mediated fatty acid uptake. Antiox Redox Signal 2013; 19(3):243-257. PMCID: PMC3691916  PDF  

Schulz TJ, Tseng YH. Brown adipose tissue: development, metabolism and beyond. Biochem J 2013; 453(2):167-78. PMCID: PMC3887508  PDF  

Cypess AM, White AP, Vernochet C, Schulz TJ, Xue R, Sass CA, Huang TL, Roberts-Toler C, Weiner LS, Sze C, Chacko AT, Deschamps LN, Herder LM, Truchan N, Glasgow AL, Holman AR, Gavrila A, Hasselgren PO, Mori MA, Molla M, Tseng YH. Anatomical localization, gene expression profiling and functional characterization of adult human neck brown fat. Nat Med 2013; 19(5):635-9. PMCID: PMC3650129  PDF

Ziogas DC, Gras-Miralles B, Mustafa S, Geiger BM, Najarian RM, Nagel JM, Flier SN, Popov Y, Tseng YH, Kokkotou E. Anti-melanin-concentrating hormone treatment attenuates chronic experimental colitis and fibrosis. Am J Physiol Gastrointest Liver Physiol 2013; 304(10):G876-84. PMCID: PMC3652072. PDF   

Schulz TJ, Huang P, Huang TL, Xue R, McDougall LE, Townsend KL, Cypess AM, Mishina Y, Gussoni E, Tseng YH. Brown-fat paucity due to impaired BMP signalling induces compensatory browning of white fat. Nature 2013; 495(7441):379-83. PMCID: PMC3623555  PDF

Liew CW, Boucher J, Cheong JK, Vernochet C, Koh HJ, Mallol C, Townsend K, Langin D, Kawamori D, Hu J, Tseng YH, Hellerstein MK, Farmer SR, Goodyear L, Doria A, Blüher M, Hsu SI, Kulkarni RN. Ablation of TRIP-Br2, a regulator of fat lipolysis, thermogenesis and oxidative metabolism, prevents diet-induced obesity and insulin resistance. Nat Med 2013; 19(2):217-26. PMCID: PMC3567215  PDF 

Müller TD, Lee SJ, Jastroch M, Kabra D, Stemmer K, Aichler M, Abplanalp B, Ananthakrishnan G, Bhardwaj N, Collins S, Divanovic S, Endele M, Finan B, Gao Y, Habegger KM, Hembree J, Heppner K, Hofmann S, Holland J, Kutschke M, Krishna R, Lehti M, Oelkrug R, Ottaway N, Perez-Tilve D, Raver C, Walch AK, Schriever S, Schroeder T, Speakman J, Tseng YH, Diaz-Meco M, Pfluger PT, Moscat J, Tschöp MH. p62 links beta-adrenergic input with mitochondrial function. J Clin Invest 2013; 123(1):469-78. PMCID: PMC3533288  PDF  

Stanford KI, Middelbeek RJW, Townsend K, Nygaard EB, An D, Hitchcox KM, Markan KR, Nakano K, Jung DY, Lee Y, Kim JK, Hirshman MF, Tseng YH, Goodyear LJ. Brown adipose tissue regulates glucose homeostasis and insulin sensitivity. J Clin Invest 2013; 123(1):215-23. PMCID: PMC3533266  PDF 

2012

Macotela Y, Emanuelli B, Mori M, Gesta S, Schulz TJ, Tseng YH, Kahn CR. Intrinsic differences in adipocyte precursor cells from different white fat depots. Diabetes 2012; 61(7):1691-9. PMCID: PMC3379665  PDF  

Townsend KL, Suzuki R, Huang TL, Jing E, Schulz TJ, Lee K, Taniguchi CM, Espinoza DO, McDougall LE, He TC, Kokkotou E, Tseng YH. BMP-7 reverses obesity and regulates appetite through a central mTOR pathway. FASEB J 2012; 26(5):2187-96. PMCID: PMC3336788. PDF    

Fujiwaraa K, Hasegawaa K, Ohkumoa T, Miyoshib H, Tseng YH, Yoshikawa K. Necdin controls proliferation of white adipocyte progenitor cells. PLoS One 2012; 7(1):e30948. PMCID: PMC3264651  PDF  

Townsend KT, Tseng YH. Brown adipose tissue: recent insights into development, metabolic function, and therapeutic potential. Adipocyte 2012; 1(1):13-24. PMCID: PMC3661118  PDF  

2011

Cypess AM, Zhang H, Schulz TJ, Huang TL, Espinoza DO, Kristiansen K, Unterman TG, Tseng YH. Insulin/IGF-I regulation of necdin and brown adipocyte differentiation via CREB- and FoxO1-associated pathways. Endocrinology 2011; 152(10):3680-9. PMCID: PMC3176640. PDF

Schleinitz D, Klöting N, Böttcher Y, Wolf S, Dietrich K, Tönjes A, Breitfeld J, Enigk B, Halbritter J, Körner A, Schön MR, Jenkner J, Tseng YH, Lohmann T, Dreßler M, Stumvoll M, Blüher M, Kovacs P. Genetic and Evolutionary Analyses of the Human Bone Morphogenetic Protein Receptor 2 (BMPR2) in the Pathophysiology of Obesity. PLoS One 2011; 6(2):e16155. PMCID: PMC3032727  PDF 

Schulz TJ, Huang TL, Tran TT, Zhang H, Townsend KL, Shadrach J, Cerletti M, McDougall LE, Giorgadze N, Tchkonia T, Schrier D, Falb D, Kirkland JL, Wagers AJ, Tseng YH. Identification of inducible brown adipocyte progenitors residing in skeletal muscle and white fat. Proc Natl Acad of Sci U S A 2011; 108(1):143-8. PMCID: PMC3017184  PDF 

2010

Zhang H, Schulz TJ, Espinoza DO, Huang TL, Emanuelli B, Kristiansen K, Tseng YH. Cross talk between insulin and bone morphogenetic protein signaling systems in brown adipogenesis. Mol Cell Bio 2010; 30(17):4224-33. PMCID: PMC2937545  PDF  

Tseng YH, Cypess AM, Kahn CR#. Cellular bioenergetics as a target for obesity therapy. Nat Rev Drug Discov 2010; 9(6):465-81. #Corresponding author. PMCID: PMC2880836  PDF  

Boucher J, Tseng YH, Kahn CR. Insulin and insulin-like growth factor-1 receptors act as ligand specific amplitude modulators of a common pathway regulating gene transcription. J Biol Chem 2010; 285(22):17235-45. PMCID: PMC2878077  PDF 

2009

Schulz TJ, Tseng YH. Emerging role of bone morphogenetic proteins in adipogenesis and energy metabolism. Cytokine Growth Factor Rev 2009: 20(5-6):523-31. PMCID: PMC2799934  PDF

Cypess AM, Lehman S, Williams G, Tal I, Rodman D, Goldfine AB, Kuo FC, Palmer EL, Tseng YH, Doria A, Kolodny GM, Kahn CR. Identification and importance of brown adipose tissue in adult humans. N Engl J Med 2009; 360(15):1509-17. PMCID: PMC2859951  PDF 

Wang WC, Chen HJ, Tseng YH, Lai YC. Identification of somatic mutations in the von Hippel-Lindau (VHL) gene in a patient with renal carcinoma. J Formos Med Assoc 2009; 108(11):886-93. PMID: 19933033. PDF 

Böttcher Y, Unbehauen H, Klöting N, Ruschke K, Körner A, Schleinitz D, Tönjes A, Enigk B, Dietrich K, Koriath M, Scholz GH, Tseng YH, Schön MR, Kiess W, Stumvoll M, Blüher M, Kovacs P. Adipose tissue expression and genetic variants of the bone morphogenetic protein receptor 1A gene (BMPR1A) are associated with human obesity. Diabetes 2009; 58(9): 2119-28. PMCID: PMC2731538  PDF

2008

Tseng YH, Kokkotou E, Schulz TJ, Huang TL, Winnay JN, Taniguchi CM, Tran TT, Suzuki R, Espinoza DO, Yamamoto Y, Ahrens MJ, Dudley AT, Norris AW, Kulkarni RN, Kahn CR. New role of bone morphogenetic protein 7 in brown adipogenesis and energy expenditure. Nature 2008; 454(7207):1000-4. PMCID: PMC2745972  PDF  

Karagiannides I, Torres D, Tseng YH, Bowe C, Carvalho E, Espinoza D, Pothoulakis C, Kokkotou E. Substance P as a novel anti-obesity target. Gastroenterology 2008; 134(3):747-55. PMCID: PMC2359157  PDF

Krüger M, Kratchmarova I, Blagoev B, Tseng YH, Kahn CR, Mann M. Dissection of the insulin signaling pathway via quantitative phosphoproteomics and knockout mouse model. Proc Natl Acad Sci U S A 2008; 105(7):2451-6. PMCID: PMC2268157  PDF  

2007

Tseng YH, He T-C. Bone morphogenetic proteins and adipocyte differentiation. Cell Science Reviews 2007; 3(3):342-60. #Corresponding author. PMCID: PMC3336788. PDF

Gesta S**, Tseng YH**, Kahn CR. Developmental origin of fat: tracking obesity to its source. Cell 2007; 131(2):242-56. **Equal authorship. PMID: 17956727. PDF

Chang L, Zhang J, Tseng YH, Xie CQ, Ilany J, Bruning J, Sun Z, Zhu X, Cui T, Youker KA, Yang Q, Kahn CR, Chen YE. Rad GTPase deficiency leads to cardiac hypertrophy. Circulation 2007; 116(25):2976-83. PMID: 18056528  PDF

Mouche S, Mkaddem SB, Wang W, Katic M, Tseng YH, Carnesecchi S, Steger K, Foti M, Meier CA, Muzzin P, Kahn CR, Ogier-Denis E, Szanto I. The NADPH oxidase NOX4 is a key mediator of adipocyte differentiation. Biochim Biophysica Acta 2007; 1773(7):1015-27. PMID: 17553579. PDF

2005

Gunton JE, Kulkarni RN, Yim S, Okada T, Hawthorne WJ, Tseng YH, Roberson RS, Ricordi C, O’Connell PJ, Gonzalez FJ, Kahn CR. Loss of ARNT/H1F1 beta mediates altered gene expression and pancreatic islet dysfunction in human type 2 diabetes. Cell 2005; 122(3):337-49. PMID: 16096055. PDF

Tseng YH, Butte AJ, Kokkotou E, Yechoor VK, Taniguchi CM, Kriauciunas KM, Cypess AL, Niinobe M, Yoshikawa K, Patti ME, Kahn CR. Prediction of preadipocyte differentiation by gene expression reveals role of insulin receptor substrates and necdin. Nat Cell Biol 2005; 7(6):601-11. PMID: 15895078. PDF

Fu M, Zhang J, Tseng YH, Cui T, Zhu X, Xiao Y, Mou Y, Leon HD, Chang M, Hamamori Y, Kahn CR, Chen YE. Rad GTPase attenuates vascular lesion formation by inhibition of vascular smooth muscle cell migration. Circulation 2005; 111(8):1071-77. PMID: 15710763. PDF